We are interested in exploring the native environment of insulin vesicles to better understand how insulin is synthesized, processed, and secreted in pancreatic ß-cells.
We use a multi-modal imaging approach to investigate insulin vesicle maturation and trafficking in response to multiple drug stimuli. We are interested in using many biophysical methods for structural characterization such as soft X-ray tomography, cryo-electron tomography, and live cell fluorescence imaging.
A significant challenge in understanding cell structure and function is integration of data from multiple experimental and computational modalities. We apply new whole-cell modeling strategies to integrate data generated from multiple experimental modalities and in different laboratories.